Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 965319
View all Tau Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human Tau T231 site
Accession # P10636

Specificity

Detects human Tau when phosphorylated at T231 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tau (T231) Antibody

Phospho-Tau (T231) antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P).

Phospho-Tau (T231) in Human Alzheimer's Brain.

Tau phosphorylated at T231 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Phospho-Tau (T231) Monoclonal Antibody (Catalog # MAB34941) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Phospho-Tau (T231) Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain

Reviewed Applications

Read 1 review rated 5 using MAB34941 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tau

Tau is a microtubule-associated protein primarily expressed in neurons. It can associate with microtubules through the carboxy-terminal domains and with the plasma membrane through the amino-terminal projection domain. Tau has a role as a stabilizer of microtubules. Abnormal Tau phosphorylation or splicing is associated with various neurological disorders.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Gene Symbol

MAPT

UniProt

P10636

Additional Tau Products

Product Documents for Phospho-Tau (T231) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Tau (T231) Antibody

For research use only

Citations for Phospho-Tau (T231) Antibody

Customer Reviews for Phospho-Tau (T231) Antibody (1)

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Customer Images

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  • Phospho-Tau (T231) Antibody
    Name: Marcel Alavi
    Application: Western Blot
    Sample Tested: Mouse Neuro2A whole cell lysate and 293T lysate
    Species: Mouse and Human
    Verified Customer | Posted 01/08/2021
    ~35 µg whole cell lysate per lane; primary AB 1:1,000 in 3% BSA TBS-T; secondary 1:5,000 AP in 5% milk powder.
    Phospho-Tau (T231) Antibody MAB34941

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Protocols

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