Detects porcine IL-2 in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human IL-2 is observed and less than 2% cross-reactivity with recombinant mouse IL-2 and recombinant rat IL-2 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant porcine IL-2 Ala21-Thr154 Accession # P26891
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
IL‑2 in Porcine PBMCs. IL‑2 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Porcine IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF652) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.
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Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.