Porcine IL-2 Biotinylated Antibody

  
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-2 in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human IL-2 is observed and less than 2% cross-reactivity with recombinant mouse IL-2 and recombinant rat IL-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant porcine IL-2
    Ala21-Thr154
    Accession # P26891
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Porcine IL‑2 (Catalog # 652-P2)
  • Immunocytochemistry
    10-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑2 in Porcine PBMCs. IL‑2 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Porcine IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF652) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma  subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma  subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.

  • References:
    1. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
    2. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
    3. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Isotype Controls
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Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Streptavidin-Alkaline Phosphatase

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NorthernLights Streptavidin NL493

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Avidin-Fluorescein

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Supplemental ELISA Products
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Streptavidin-HRP

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EvenCoat Streptavidin Coated Plates

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EvenCoat Streptavidin Coated Plates, 5 Pack

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