Detects porcine IL‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human IL-2 is observed and less than 1% cross-reactivity with recombinant mouse IL-2 and recombinant rat IL-2 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant porcine IL-2 Ala21-Thr154 Accession # P26891
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A. J. H. and C. B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.25-0.8 µg/mL in the presence of 2 ng/mL Recombinant Porcine IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑2 and Neutralization by Porcine IL‑2 Antibody. Recombinant Porcine IL‑2 (Catalog # 652-P2) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF652). The ND50 is typically 0.25-0.8 µg/mL.
IL‑2 in Porcine PBMCs. IL‑2 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Goat Anti-Porcine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF652) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.
Taniguchi, T. and Y. Minami (1993) Cell 73:5.
Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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