Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha 2u-Globulin Primary Antibodies »

Product Specifications

Immunogen

Rat urine-derived alpha 2u-Globulin

Specificity

Detects rat alpha 2u-Globulin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat alpha 2u‑Globulin Antibody

a2u-Globulin antibody in Rat Liver by Immunohistochemistry (IHC-Fr).

alpha 2u-Globulin in Rat Liver.

a2u-Globulin was detected in perfusion fixed frozen sections of rat liver using 5 µg/mL Goat Anti-Rat a2u-Globulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF586) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat alpha 2u‑Globulin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver

Western Blot

0.1 µg/mL
Sample: Rat alpha 2u-Globulin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 2u-Globulin

Rat alpha 2u-Globulin (AUG), also known as major urinary protein, belongs to the lipocalin protein family. Under complex hormonal control, alpha 2u-globulin is a low molecular weight protein expressed in a tissue-, age-, and sex-specific fashion. Circulating alpha 2u-globulin is expressed by the liver. It is removed from the plasma by the kidney and excreted.

Rodent studies have employed alpha 2u-globulin nephropathy as an experimental model for the development of kidney tumors. Induction of alpha 2u-globulin nephropathy alters the number, size and shape of hyaline droplets in the proximal tubules. Intra-cellular accumulation of alpha 2u-globulin induces cell death and necrosis of tubular epithelial cells.

Alternate Names

AUG, PGCL1

Additional alpha 2u-Globulin Products

Product Documents for Rat alpha 2u‑Globulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat alpha 2u‑Globulin Antibody

For research use only

Related Research Areas

Diabetes
Obesity

Citations for Rat alpha 2u‑Globulin Antibody

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Protocols

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