Rat B7-1/CD80 Antibody

Catalog # Availability Size / Price Qty
MAB1214
MAB1214-SP
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Rat B7-1/CD80 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat B7‑1/CD80 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human B7-1, recombinant mouse (rm) B7-1, rmB7-2, recombinant rat B7-2, rmB7-H1, rmB7-H2, rmB7-H3, or rmPD-L2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 210408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat B7‑1/CD80
Ile39-Gln248
Accession # AAB60503
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat B7‑1/CD80 Fc Chimera (Catalog # 1214-B7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

It is widely accepted that T cells require a signal induced by the engagement of the T cell receptor and a co-stimulatory signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co-stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3 and PD-L2 (1). B7-1 is expressed on activated B cells, activated T cells, mature dendritic cells and macrophages (2). T cells express two different receptors capable of binding B7-1, CD28 and CTLA-4. CTLA-4 binds to B7-1 with a 20 - 100 fold higher affinity than CD28. Engagement of CD28 by B7-1 increases T cell proliferation and IL-2 production and enhances the immune response (3). In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response (4). Rat B7-1 is a 320 amino acid (aa) protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Rat and human B7-1 share 47% amino acid identity.

References
  1. Coyle, A.J. and J-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
  2. Azuma, M. et al. (1992) Nature 366:76.
  3. Gimmi, C.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88:6575.
  4. Walunas, T.L. et al. (1994) Immunity 1:405.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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