Detects rat B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat B7-1, recombinant human B7-2, recombinant mouse (rm) B7-2, rmB7-H1, rmB7-H2, rmB7-H3, or rmPD‑L is observed.
Monoclonal Mouse IgG1 Clone # 199622
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat B7‑2/CD86 Met1-Ile250 Accession # NP_064466
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize B7‑2/CD86 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 1 µg/mL Recombinant Rat B7‑2/CD86 Fc Chimera and 10 µg/mL PHA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑2 Secretion Induced by B7‑2/CD86 and Neutralization by Rat B7‑2/CD86 Antibody.
Recombinant Rat B7‑2/CD86 Fc Chimera (Catalog # 1340-B2) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Rat B7‑2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7‑2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND50 is typically 0.3‑1.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
B7-2/CD86 and the related B7-1/CD80 are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. B7-2 is highly expressed on activated antigen presenting cells and vascular endothelial cells. It binds to CD28 on T cells to enhance T cell activation. It additionally binds CTLA-4 which inhibits the T cell response and supports regulatory T cell function. The relative participation of B7-1 and B7-2 in T cell co-stimulation can alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses.
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