Detects rat C‑Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CRP is observed and 10% cross-reactivity with recombinant human CRP is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant rat C-Reactive Protein/CRP His20-Ser230 Accession # P48199
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Rat C‑Reactive Protein/CRP by Western Blot.
Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for C‑Reactive Protein/CRP at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
C-Reactive Protein/CRP in Rat Liver.
C-Reactive Protein/CRP was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Rat C‑Reactive Protein/CRP by Simple WesternTM.
Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for C‑Reactive Protein/CRP at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Rat C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. The rat CRP gene encodes a 230 amino acid (aa) precursor with a signal peptide of 19 aa and the mature polypeptide of 211 aa. Rat CRP shares 64% and 70% aa sequence homology with human and mouse CRP respectively. Human, mouse and rabbit CRP are non-glycosylated proteins, and the units are non-covalently linked to form the pentameter. In contrast, rat CRP is a glycoprotein and contains a covalently linked dimer in the pentameter. CRP exhibits Ca++-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, the membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phagocytotic responses. In addition to phagocytosis, CRP also induces the production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF-alpha. In human and rabbits, CRP is an important acute-phase protein that plays a role in the first line of host innate defense. The level of plasma CRP at basal conditions in human and rabbits is very low, and can increase 1,000-fold within 24‑48 hours in response to infection, inflammation or tissue damage. In rats, CRP exists at a high level at basal conditions and only increases about 2-fold in response to injury. CRP is not a typical acute-phase protein in rat and is a minor component in response to injury. In mice, CRP is expressed at very low levels and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is an acute phase serum protein in mice.
Mohammad, R. et al. (1992) J. Biol. Chem. 267:2947.
Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
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