Rat CD25/IL-2R alpha Antibody

Catalog # Availability Size / Price Qty
AF5156
AF5156-SP

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Rat CD25/IL-2R alpha Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant mouse CD25/IL-2 R alpha is observed, approximately 10% cross-reactivity with recombinant human (rh) CD25/IL-2 R alpha is observed, and less than 5% cross-reactivity with rhIL-2 R beta is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant rat CD25/IL-2 R alpha
Glu22-Gln235
Accession # P26897
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM)
Immunocytochemistry
5-15 µg/mL
Immersion fixed rat splenocytes activated with 1 ug/mL PHA for 3 days

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The rat IL-2 R alpha cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aa signal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4). Within the ECD, rat IL-2 R alpha shares 58% and 81% aa sequence identity with human and mouse IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and  gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11‑13).

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
  4. Page, T.H. and M.J. Dallman (1991) Eur. J. Immunol. 21:2133.
  5. Hatakeyama, M. et al. (1989) Science 244:551.
  6. Takeshita, T. et al. (1992) Science 257:379.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  10. Witkowska, A.M. (2005) Mediators Inflamm. 2005:121.
  11. Willerford, D.M. et al. (1995) Immunity 3:521.
  12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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