Detects rat GM-CSF in direct ELISAs and Western blot. In direct ELISAs, approximately 100% cross-reactivity with recombinant feline GM-CSF and recombinant porcine GM-CSF is observed, approximately 30% cross-reactivity with recombinant mouse GM‑CSF, recombinant canine GM-CSF and recombinant human GM‑CSF is observed.
Polyclonal Goat IgG
E. coli-derived recombinant rat GM-CSF (R&D Systems, Catalog # 518-GM) Ala1-Lys127 Accession # P48750
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 ug/mL in the presence of 0.25 ng/mL Recombinant Rat GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by GM‑CSF and Neutralization by Rat GM‑CSF Antibody.
Recombinant Rat GM‑CSF induces proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by rrGM‑CSF is neutralized (green line) by increasing concentrations of Goat Anti-Rat GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF518). The ND50 is typically 0.1-0.6 ug/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices. Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha. Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells.
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