Rat ICAM-1/CD54 Antibody Summary
Accession # Q00238
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB583) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Citations for Rat ICAM-1/CD54 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
Authors: Li PC, Chen WC, Chang LC, Lin SC
Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L912-20.
Sample Types: Tissue Homogenates
Applications: Western Blot
Cell-based angiopoietin-1 gene therapy for acute lung injury.
Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
Sample Types: Cell Lysates
Applications: Western Blot
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