Rat IL-18/IL-1F4 Antibody

Catalog # Availability Size / Price Qty
AF521
AF521-SP
IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Rat IL‑18/IL‑1F4 Antibody.
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Citations (5)
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Rat IL-18/IL-1F4 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL‑18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse IL‑18 is observed and approximately 10% cross-reactivity with recombinant human IL‑18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IL‑18/IL-1F4
His37-Ser194
Accession # P97636
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521-RL)
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells [Ahn, H.J. et al. (1997) J. Immunol. 159:2125]. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Rat IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Rat IL‑18/IL‑1F4 Antibody. View Larger

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Rat IL‑18/IL‑1F4 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419‑ML), Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521‑RL) stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Rat IL‑18/IL‑1F4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF521). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18/IL-1F4

Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rat IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60-64% aa sequence identity with human, canine, feline, porcine, and rhesus macaque IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).

References
  1. Arend, W.P. et al. (2008) Immunol. Rev. 223:20.
  2. Culhane, A.C. et al. (1998) Mol. Psychiatry 3:362.
  3. Ghayur, T. et al. (1997) Nature 386:619.
  4. Gu, Y. et al. (1997) Science 275:206.
  5. Boraschi, D. and C.A. Dinarello (2006) Eur. Cytokine Netw. 17:224.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  8. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  9. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  10. Takeda, K. et al. (1998) Immunity 8:383.
  11. Park, S. et al. (2007) Cell. Mol. Immunol. 4:329.
  12. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  13. Hoshino, T. et al. (2001) J. Immunol. 166:7014.
  14. Iannello, A. et al. (2009) AIDS Rev. 11:115.
  15. Rabkin, S.W. (2009) Nat. Clin. Pract. Cardiovasc. Med. 6:192.
  16. Netea, M.G. et al. (2006) Nat. Med. 12:650.
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Product Datasheets

Citations for Rat IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain
    Authors: S Kuwahara-O, S Maeda, K Kobayashi, Y Minato, K Tanaka, K Yamanishi, M Hata, W Li, T Hayakawa, K Noguchi, H Okamura, H Yagi
    Neurosci. Lett., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
    Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
    J. Neurosci., 2008;28(48):12775-87.
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo
  3. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Neut
  4. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
    Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
    Ann. Neurol., 2005;57(1):50-9.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  5. Role of IL-18 in acute lung inflammation.
    Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
    J. Immunol., 2001;167(12):7060-8.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development

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Rat IL‑18/IL‑1F4 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Rat,spinal cord dorsal horn, particle nerve injury (CCI) Species: Rat

Fixed with 4%Polyoxymethylene and 15%saturation picric acid. Without antigen retrieval.
Frozen section, 20m, floating slice method.
First antibody 4 degrees over night and secondary antibody 2 hours at room-temperature.
Antigen retrieval in 99 degrees citrate buffer(pH 4.6) for 5min would benefit the output (not tested).
Green: Interleukin-18; red: Iba-1 (microglia marker).
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1/100