Rat SMAGP Antibody

Catalog # Availability Size / Price Qty
AF3967
AF3967-SP
Product Details
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Rat SMAGP Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human SMAGP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat SMAGP
Met1-Thr34, Phe56-Ile97
Accession # Q7TPF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat SMAGP
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of rat pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAGP

SMAGP (small transmembrane and glycosylated protein) is a 25 kDa, type III transmembrane glycoprotein that participates in epithelial cell polarity. Rat SMAGP is 97 amino acids (aa) in length and contains no signal sequence. The N-terminal 34 amino acids constitute the extracellular domain and show O-linked glycosylation. The C‑terminal 42 amino acids (56‑97) make up the cytoplasmic region. SMAGP is likely involved in cell adhesion and communication. Relocation of SMAGP from the membrane to the cytosol is associated with tissue disorganization and metastatic transformation. Over the extracellular and cytoplasmic domains, rat SMAGP shares 96% and 66% aa sequence identity with mouse and human SMAGP, respectively.

Long Name
Small Transmembrane and Glycosylated Protein
Entrez Gene IDs
57228 (Human); 207818 (Mouse); 300236 (Rat)
Alternate Names
hSMAGP; LOC57228; MGC149453; MGC149454; SMAGP; small cell adhesion glycoprotein; small cell transmembrane and glycosylated protein; Small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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