Recombinant Human EGF GMP Protein, CF

Name: Recombinant Human EGF GMP Protein, CF
Brand: R&D Systems
SKU: 236-GMP
Rating: 5 (2 reviews)

Catalog #: 236-GMP
3 Citations 2 Reviews Datasheet / COA / SDS

Animal-Free

Catalog #	Availability	Size / Price	Qty
236-GMP-01M
236-GMP-200

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Recombinant Human EGF GMP Protein Bioactivity

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All EGF Proteins and Enzymes

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EGF GMP Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. The result of the major peak from mass spectrometry analysis is 6347 Da, which corresponds to the calculated molecular mass of 6353 Da

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 20‑100 pg/mL.
The specific activity of Recombinant Human EGF is >8.0 x 10⁵ IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).

Source

E. coli-derived human EGF protein
Asn971-Arg1023, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Accession #

P01133.2

N-terminal Sequence
Analysis

Met-Asn₉₇₁-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser

Predicted Molecular Mass

6 kDa

SDS-PAGE

6 kDa, reducing conditions

Product Datasheets

236-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

236-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human EGF (Catalog # 236‑GMP) stimulates proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 20‑100 pg/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP EGF.

SDS-PAGE

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1 µg/lane of GMP-grade Recombinant Human EGF (Catalog # 236-GMP) was resolved by SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Mass Spectrometry

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MALDI‑TOF analysis of GMP‑grade Recombinant Human EGF (Catalog # 236‑GMP). The major peak corresponds to the calculated molecular mass, 6353 Da. The minor peak at 6549 Da is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Background: EGF

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References

Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented and controlled manufacturing process
Control of documentation and process changes by QA
Personnel training programs
Raw material inspection and vendor qualification/monitoring program
Validated equipment, processes and test methods
Equipment calibration and maintenance schedules using a Regulatory Asset Manager
Facility/Utilities maintenance, contamination controls, safety and pest control programs
Material review process for variances
Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

N-terminal amino acid analysis
SDS-PAGE purity analysis
Molecular weight analysis via mass spectrometry
Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
Bioassay analysis
Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
Host cell protein assessment
Host cell DNA assessment
Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers.

Please read our complete Animal-Free Statement

Product Specific Notices

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

Related Research Areas

Citations for Recombinant Human EGF GMP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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miR-3174 Is a New Tumor Suppressor MicroRNA That Inhibits Several Tumor-Promoting Genes in Glioblastoma
Authors: Hanif, F;Zhang, Y;Dube, C;Gibert, MK;Saha, S;Hudson, K;Marcinkiewicz, P;Kefas, B;Guessous, F;Abounader, R;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Neural stem cell transplantation in patients with progressive multiple sclerosis: an open-label, phase 1 study
Authors: A Genchi, E Brambilla, F Sangalli, M Radaelli, M Bacigalupp, R Furlan, A Andolfo, D Drago, C Magagnotti, GM Scotti, R Greco, P Vezzulli, L Ottoboni, M Bonopane, D Capilupo, F Ruffini, D Belotti, B Cabiati, S Cesana, G Matera, L Leocani, V Martinelli, L Moiola, L Vago, P Panina-Bor, A Falini, F Ciceri, A Uglietti, MP Sormani, G Comi, MA Battaglia, MA Rocca, L Storelli, E Pagani, G Gaipa, G Martino
Nature Medicine, 2023-01-09;29(1):75-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis
Authors: CJ Brereton, L Yao, ER Davies, Y Zhou, M Vukmirovic, JA Bell, S Wang, RA Ridley, LSN Dean, OG Andriotis, F Conforti, L Brewitz, S Mohammed, T Wallis, A Tavassoli, RM Ewing, A Alzetani, BG Marshall, SV Fletcher, PJ Thurner, A Fabre, N Kaminski, L Richeldi, A Bhaskar, CJ Schofield, M Loxham, DE Davies, Y Wang, MG Jones
Elife, 2022-02-21;11(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay