Human NKX2.5 Antibody

Catalog # Availability Size / Price Qty
AF2444
AF2444-SP
Product Details
Citations (4)
FAQs
Supplemental Products
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Human NKX2.5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKX2.5 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NKX2.5
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human heart

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX2.5

The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm and plays an essential role in heart development.

Long Name
NK2 Homeobox 5
Entrez Gene IDs
1482 (Human)
Alternate Names
cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5; NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog

Product Datasheets

Citations for Human NKX2.5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016;11(11):e0165926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC - Fresh
  2. Cobalt protoporphyrin pretreatment protects human embryonic stem cell-derived cardiomyocytes from hypoxia/reoxygenation injury in vitro and increases graft size and vascularization in vivo.
    Authors: Luo J, Weaver M, Cao B, Dennis J, Van Biber B, Laflamme M, Allen M
    Stem Cells Transl Med, 2014;3(6):734-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  3. Surgical models for cardiac regeneration in neonatal mice.
    Authors: Mahmoud, Ahmed I, Porrello, Enzo R, Kimura, Wataru, Olson, Eric N, Sadek, Hesham A
    Nat Protoc, 2014;9(2):305-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  4. Scaffold-free human cardiac tissue patch created from embryonic stem cells.
    Authors: Stevens KR, Pabon L, Muskheli V
    Tissue Eng Part A, 2009;15(6):1211-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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