Human NKX2.5 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (5)
Supplemental Products

Human NKX2.5 Antibody Summary

Species Reactivity
Detects human NKX2.5 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human NKX2.5
Accession # P52952
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human NKX2.5
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human heart

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX2.5

The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm and plays an essential role in heart development.

Long Name
NK2 Homeobox 5
Entrez Gene IDs
1482 (Human)
Alternate Names
cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5; NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog

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Citations for Human NKX2.5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction
    Authors: W Chen, D Pretorius, Y Zhou, Y Nakada, J Yang, J Zhang
    JCI Insight, 2021;6(20):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: ICC
  2. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016;11(11):e0165926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  3. Cobalt protoporphyrin pretreatment protects human embryonic stem cell-derived cardiomyocytes from hypoxia/reoxygenation injury in vitro and increases graft size and vascularization in vivo.
    Authors: Luo J, Weaver M, Cao B, Dennis J, Van Biber B, Laflamme M, Allen M
    Stem Cells Transl Med, 2014;3(6):734-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. Surgical models for cardiac regeneration in neonatal mice.
    Authors: Mahmoud, Ahmed I, Porrello, Enzo R, Kimura, Wataru, Olson, Eric N, Sadek, Hesham A
    Nat Protoc, 2014;9(2):305-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Scaffold-free human cardiac tissue patch created from embryonic stem cells.
    Authors: Stevens KR, Pabon L, Muskheli V
    Tissue Eng Part A, 2009;15(6):1211-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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