Culture media specifically optimized for use with hematopoietic stem cells.
Researchers employing stem or progenitor cells in their studies currently use a wide variety of media products to support their cultures. These media are generally developed or validated for cells other than stem cells, thus making them less desirable than cell type-specific media. To address this need, R&D Systems offers StemXVivo media products specifically designed for stem/progenitor cell culture. All the components included within these defined, basal, serum-free formulations have been selected, optimized, and validated for hematopoietic stem cell culture.
Serum-Free Dendritic Cell Base Media (Catalog # CCM003)
Base Media optimized for the culture and differentiation of human dendritic cells.
Serum-Free T Cell Base Media (Catalog # CCM010)
Base Media optimized for the ex vivo culture of human T lymphocytes.
|Dendritic Cell Culture and Maturation from CD14+ Monocytes. A. LPS-matured monocyte-derived dendritic cells were obtained after a 9-day culture of CD14-enriched monocytes in StemXVivo Serum-Free Dendritic Cell Base Media (Catalog # CCM003) supplemented with GM-CSF (Catalog # 215-GM) and IL-4 (Catalog # 204-IL). B. Dendritic cells cultured for 7 days were stained with the mature dendritic cell marker CD83 before (gray line) and after a two day treatment with LPS (black line). Isotype-matched control staining is also shown (filled histogram).|