Sleeping Beauty Transposase Antibody

(3 citations)   
  • Specificity
    Detects Sleeping Beauty (SB) Transposase in Western blots. In Immunohistochemistry tests, this antibody stains SB transposase transgenic mouse tissue sections but not wild type mouse tissue sections.
  • Source
    Monoclonal Mouse IgG1 Clone # 324622
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant Sleeping Beauty Transposase
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Sleeping Beauty Transposase
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Sleeping Beauty Transposase in Mouse Liver. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Sleeping Beauty Transposase in Mouse Intestine. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sleeping Beauty Transposase
SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired-like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).
  • References:
    1. Ivics, Z. et al. (1997) Cell 91:501.
  • Alternate Names:
    SB Transposase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. A transposon-based analysis reveals RASA1 is involved in triple negative breast cancer
    Authors: C Su rez-Cab, RM Quintana, A Bravo, ML Casanova, A Page, JP Alameda, JM Paramio, A Maroto, J Salamanca, AJ Dupuy, A Ram¡rez, M Navarro
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Transposon mutagenesis identifies genes and cellular processes driving epithelial-mesenchymal transition in hepatocellular carcinoma
    Authors: Takahiro Kodama
    Proc Natl Acad Sci USA, 2016;113(24):E3384-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Sleeping beauty transposition from nonintegrating lentivirus.
    Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
    Mol. Ther., 2009;17(7):1197-204.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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