STAT3 [p Tyr705] Antibody - BSA Free

Name: STAT3 [p Tyr705] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-19129
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-19129

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This affinity purified STAT3 [p Tyr705] Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human STAT3. (Uniprot: P40763)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Modification

p Tyr705

Specificity

Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide

Store vial at-20C prior to opening. Aliquot contents and freeze at -20C or below forextended storage. Avoid cycles of freezing and thawing. Centrifuge product ifnot completely clear after standing at room temperature. This product is stablefor several weeks at 4C as an undiluted liquid. Dilute only prior toimmediate use.

Scientific Data Images for STAT3 [p Tyr705] Antibody - BSA Free

Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129]

Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129] - Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 ug per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 (R) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NBP2-19129]

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NBP2-19129] - Rabbit anti STAT3 pY705 Antibody at 40X in colon tissue at pH 9. Tissue: Human Colon.

STAT3 [p Tyr705] Antibody

Immunohistochemistry of Rabbit anti STAT3 [p Tyr705] Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for STAT3 [p Tyr705] Antibody - BSA Free

Application

Recommended Usage

ELISA

1:10000-1:30000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:250

Western Blot

1 ug/ml

Application Notes

This product has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: STAT3

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Gene Symbol

STAT3

UniProt

P40763

Additional STAT3 Products

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Product Specific Notices for STAT3 [p Tyr705] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.