Viral Dengue Virus NS1 Antibody

Catalog # Availability Size / Price Qty
MAB94442-100
MAB94442-SP
Dengue Virus NS1 ELISA Standard Curve.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Viral Dengue Virus NS1 Antibody Summary

Species Reactivity
Viral
Specificity
In sandwich immunoassays, Dengue Virus 1/2/3/4 NS1 is detected.
Source
Monoclonal Mouse IgG2A Clone # 971332
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 4 NS1 protein
Asp1-Ala352
Accession # NP_740318
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94441).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Dengue Virus NS1 ELISA Standard Curve. Recombinant Dengue Virus 1 NS1, Recombinant Dengue Virus 2 NS1, Recombinant Dengue Virus 3 NS1, or Recombinant Dengue Virus 4 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94441) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name
Dengue Virus Nauru/West Pac/1974 Polyprotein
Alternate Names
Dengue NS1 protein; Dengue NS1; Dengue Virus NS1; Dengue Virus

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Viral Dengue Virus NS1 Antibody

There are currently no reviews for this product. Be the first to review Viral Dengue Virus NS1 Antibody and earn rewards!

Have you used Viral Dengue Virus NS1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review