Key Product Details

Species Reactivity

Viral

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 462510
View all HHV8-ORF74 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with viral HHV8-ORF74
Met1-Thr342
Accession # AAB51506

Specificity

Detects viral HHV8-ORF74.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Viral HHV8‑ORF74 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with viral HHV8-ORF74

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HHV8-ORF74

HHV8‑ORF74 is a 342 amino acid viral 7-transmembrane receptor encoded by the Kaposi's sarcoma-associated herpesvirus 8. The G‑protein-coupled receptor is a constitutively active homologue of CXCR2, the CXCL8 receptor (IL-8 RB). It contributes to Kaposi's sarcoma by triggering expression of paracrine factors such as VEGF-A, inflammatory cytokines, adhesion molecules and chemokines, and enhancing cell growth.

Long Name

Human Herpesvirus 8 ORF74

Alternate Names

HHV8ORF74, KSHV-vGPCR

Entrez Gene IDs

4961460 (Viral)

Gene Symbol

HHV8GK18_GP84

UniProt

AAB51506

Additional HHV8-ORF74 Products

Product Documents for Viral HHV8‑ORF74 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Viral HHV8‑ORF74 Antibody

For research use only

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