Viral wnvNS1 Protein Antibody Summary
Accession # NP_776015
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290722).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Viral wnvNS1 Protein ELISA Standard Curve. Recombinant Viral wnvNS1 Protein protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290722) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290721) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: wnvNS1 Protein
West Nile Virus, or WNV, is a member of the Flaviviridae family that includes other human pathogens such as yellow fever (YF), dengue (DEN), tick-borne encephalitis (TBE), Japanese encephalitis (JEV), and Murray Valley encephalitis (MVE) viruses. West Nile virus (WNV) is a single-stranded RNA mosquito-transmitted virus that causes epidemics of encephalitis and viscerotropic disease. The WNV nonstructural protein NS1 is a 48-kDa highly conserved secreted glycoprotein that does not package with the virion. Even when absent from the virion, immunization with NS1 typically elicits a protective immune response.
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