Immune Checkpoint Blockade: Blocking Antibodies

Blockade of immune checkpoint molecules, such as CTLA-4 and PD-1/PD-L1, is a promising therapeutic approach for cancer treatment. Unfortunately, blocking CTLA-4 or PD-1/PD-L1 is not universally successful across different cancer types and patients. It is thought that co-targeting multiple checkpoint molecules simultaneously could improve the effectiveness of this strategy. Take advantage of the best blocking antibodies on the market to identify the next key immune checkpoint target.

Immune Checkpoint Blockade Homepage

R&D Systems® Blocking Antibodies

The selection and quality of our blocking antibodies is unparalleled. All of our blocking antibodies are tested in relevant bioassays and manufactured to ensure low endotoxin levels so you can always be confident in their performance. Learn more about what makes our blocking antibodies the best. Click the boxes below to view our selection of blocking antibodies for each molecule listed.


CTLA-4  PD-1   PD-L1   PD-L2   B7-H3  B7-H7/HHLA2  M-CSF


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CTLA-4 Inhibition of B7-1/CD80-induced IL-2 Secretion and Neutralization by Mouse CTLA-4 Antibody.
Recombinant Mouse CTLA-4 Fc Chimera (Catalog # 434-CT) inhibits Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine® ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7-1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA-4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA-4 Monoclonal Antibody (Catalog # MAB434). The ND50 is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL).

Selected Citations

Our blocking antibodies have been cited in thousands of publications. Use the antibodies that are trusted by your peers.

IL-17 Antibody (Catalog # MAB421)
Qi, H. et al. (2015) Immunobiology 220:54.
CTLA-4 Antibody (Catalog # MAB434)
Cunnusamy, K. et al. (2011) J. Immunol. 186:6737.
Inoue, S. et al. (2011) Shock 36:38.
IL-12/IL-35 p35 Antibody (Catalog # MAB1570)
Rossetti, M. et al. (2015) J. Immunol. 194:113.
PD-1 Antibody (Catalog # AF1086)
Kondo, A. et al. (2010) Blood 116:1124.
CCL4/MIP-1 beta Antibody (Catalog # MAB271)
Cardone, M. et al. (2015) J. Virol. 89:4798.

Explore More Products for T Cell Co-Signaling Molecules

Download the brochure below and explore our interactive pathway for an informative graphical outline of T cell co-stimulatory and co-inhibitory molecules and to learn about our extensive offering of antibodies, proteins, and ELISAs for this area of research.

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