Home / BoNT-D Heavy Chain / C. botulinum BoNT-D Heavy Chain Antibody

C. botulinum BoNT-D Heavy Chain Antibody

Catalog #: MAB6639 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6639
MAB6639-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-D Heavy Chain Antibody Summary

Species Reactivity
C. botulinum
Specificity
Detects recombinant C. botulinum BoNT-D Heavy Chain in direct ELISAs and Western blots. 100% and approximately 10% cross-reactivity with recombinant C. botulinum BoNT-C1 Heavy Chain is observed in Western blots and direct ELISAs, respectively. In direct ELISAs, no cross-reactivity with the Heavy Chains of BoNT-A, -E, or -G or the Light Chains of BoNT-A, -B, -C1, -D, -E, -F, -G is observed.
Source
Monoclonal Mouse IgG2B Clone # 682810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant Clostridium botulinum Neurotoxin Type D Heavy Chain
Asn862-Glu1276
Accession # P19321
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant C. botulinum BoNT-D Heavy Chain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-D Heavy Chain

BoNT-D (Botulinum neurotoxin serotype D) is produced by Clostridium botulinum and inhibits acetylcholine release from neuromuscular junctions. Notably, BoNT-D is not toxic to human cells. The 1276 amino acid (aa) BoNT-D precursor is cleaved to generate a disulfide-linked dimer of the 50‑55 kDa light chain peptidase and the 100 kDa heavy chain. The heavy chain contains receptor binding and pore forming domains. The light chain translocates through this pore to the target cell cytosol where it cleaves synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Within aa 862-1276 of the heavy chain, BoNT-D shares 31%, 33%, and 41% aa sequence identity with BoNT-A, -B, and -C1 heavy chains, respectively.

Long Name
Botulinum Neurotoxin Type D Heavy Chain
Alternate Names
BoNTD Heavy Chain; BoNT-D Heavy Chain

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