Key Product Details

Species Reactivity

C. botulinum

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 682810
View all BoNT-D Heavy Chain Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Clostridium botulinum Neurotoxin Type D Heavy Chain
Asn862-Glu1276
Accession # P19321

Specificity

Detects recombinant C. botulinum BoNT-D Heavy Chain in direct ELISAs and Western blots. 100% and approximately 10% cross-reactivity with recombinant C. botulinum BoNT-C1 Heavy Chain is observed in Western blots and direct ELISAs, respectively. In direct ELISAs, no cross-reactivity with the Heavy Chains of BoNT-A, -E, or -G or the Light Chains of BoNT-A, -B, -C1, -D, -E, -F, -G is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for C. botulinum BoNT‑D Heavy Chain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant C. botulinum BoNT-D Heavy Chain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BoNT-D Heavy Chain

BoNT-D (Botulinum neurotoxin serotype D) is produced by Clostridium botulinum and inhibits acetylcholine release from neuromuscular junctions. Notably, BoNT-D is not toxic to human cells. The 1276 amino acid (aa) BoNT-D precursor is cleaved to generate a disulfide-linked dimer of the 50‑55 kDa light chain peptidase and the 100 kDa heavy chain. The heavy chain contains receptor binding and pore forming domains. The light chain translocates through this pore to the target cell cytosol where it cleaves synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Within aa 862-1276 of the heavy chain, BoNT-D shares 31%, 33%, and 41% aa sequence identity with BoNT-A, -B, and -C1 heavy chains, respectively.

Long Name

Botulinum Neurotoxin Type D Heavy Chain

Alternate Names

BoNTD Heavy Chain

UniProt

P19321 (C. botulinum)

Additional BoNT-D Heavy Chain Products

Product Documents for C. botulinum BoNT‑D Heavy Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for C. botulinum BoNT‑D Heavy Chain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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