Canine IL-3 Antibody Summary
Accession # Q9BDX4
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑3 in Canine PBMCs. IL-3 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-3 Monoclonal Antibody (Catalog # MAB6658) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals.
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