Cell Culture Media

R&D Systems™ cell culture media are designed to support the in vitro cultivation of cell lines, primary mammalian cells, and advanced cell culture models. With over 30 years of experience, each production lot of cell culture media is thoroughly tested to ensure the superior quality and lot-to-lot consistency that researchers worldwide expect from our highly bioactive recombinant proteins.

ExCellerate Cell Expansion Media provide an optimized serum-free culture environment for the generation and expansion of individual immune cell types and induced pluripotent stem cells (iPSCs).

Robust expansion and maintenance of cell cultures
Reduce variability in your cell cultures
Streamline comparability testing if raw material changes are required
Cross-reference Drug Master Files to facilitate regulatory compliance
Reduce time-to-market for your cell therapy

Immune Cell Expansion Media

Mitigating risk and embedding flexibility into your cell culture process will improve process reproducibility and accelerate your immune cell therapy development. These ExCellerate cell expansion media are optimized for specific cell types and are key to building a culture process that ensures high yields and high quality.

ExCellerate Human NK Cell Expansion Media

Made from completely animal-free raw materials
Serum-free and phenol red-free formulation
Maintains high fold expansion and killing capacity
Enables restimulation without exhaustion

View Human NK Cell Expansion Media

Excellerate Human NK Cell Expansion Media

GMP Human T Cell Media

Clinic-ready formulation
Robust T cell expansion from PBMCs or purified T cells
Adopted by academic and biopharma clinical programs

View GMP T Cell Media

Supplement With GMP Human AB Serum

GMP Human T Cell Media

Pluripotent Stem Cell Expansion Media

Achieve robust expansion and extended maintenance of your stem cells in well-defined culture media. ExCellerate iPSC Expansion Media sustains cell viability, genotype, and multi-lineage differentiation potential/pluripotency over long term culture and is compatible with various matrices and cell lines. Seamlessly transition to clinical applications with equivalent preclinical RUO and GMP-grade media.

ExCellerate GMP and Preclinical RUO iPSC Expansion Media

Animal component-free, all-in-one formulation
Compatible with a variety of matrices and cell lines
Maintains stable cell integrity over long-term culture
GMP media is manufactured in an animal-free facility with ISO:9001 and ISO:13485 certifications

View GMP iPSC Media View RUO iPSC Media

Available Media and Supplements

Immune Cell Expansion Media
ExCellerate B Cell Media	CCM031 (Xeno-Free)
ExCellerate NK Cell Expansion Media	CCM032 (Xeno-Free)	CCM037 (Animal Component-Free)
Human T Cell Expansion Media	CCM030 (Xeno-Free)	CCM038-GMP-1B (GMP, Bag)	CCM038-GMP-1L (GMP, Bottle)

Hematopoietic Stem Cell Media
Human Methylcellulose Media	HSC002 (Base Media)	HSC003 (Complete Media)	HSC004 (Complete, Without Epo)
Human Methylcellulose Media	HSC005 (Enriched Media)	HSC005SF (Enriched, Serum-Free)
Mouse Methylcellulose Media	HSC006 (Base Media)	HSC007 (Complete Media)

Stem Cell Differentiation and Expansion Media
ExCellerate iPSC Expansion Media	CCM036 (Animal Component-Free)	CCM036-GMP (GMP, Animal-Free)
Endothelial Cell Growth Media	CCM027
Mouse Embryonic Fibroblast (MEF) Conditioned Media	AR005
StemXVivo Chondrogenic Base Media	CCM005
StemXVivo Dendritic Cell Base Media	CCM003 (Serum-Free)
StemXVivo MSC Expansion Media	CCM004	CCM014 (Serum-Free)	CCM021 (Xeno-Free)
StemXVivo MSC Freezing Media	CCM016 (Serum-Free)
StemXVivo Osteogenic/Adipogenic Base Media	CCM007

Media Supplements
Human AB Serum	HABS001-GMP-100ML (Human AB Serum)
N-2 Plus N-2 MAX	AR003 (Contains Bovine Insulin)	AR009 (Contains Human Insulin)	AR016 (GMP, Xeno-Free)
N21-MAX	AR008	AR010 (Insulin-Free)	AR012 (Vitamin A-Free)
Hematopoietic Stem Cell	HSC001 (Methylcellulose Stock Solution)	HSC011 (Methylcellulose Concentrate)
Stem Cell	CCM006 (Chondrogenic)	CCM008 (Human Osteogenic)	CCM009 (Mouse/Rat Osteogenic)
Stem Cell	CCM011 (Adipogenic)	CCM017 (EMT-Inducing)

N-2 and N21-MAX Media Supplements

N-2 media supplements are optimized for neural progenitor cells and their differentiated derivatives. Improved over published N1 formulation.

N21-MAX standard and insulin-free media supplements are optimized for neuronal cell cultures. Vitamin A-free is optimized for neural cell cultures and limits retinoic acid production to reduce unwanted differentiation. Improved over published B27/NS21 formulation.

Xeno-Free vs. Animal Component-Free Culture Media

Xeno-free media do not contain any non-human animal-derived components. Xeno-free media may contain human-derived components such as serum, holo-transferrin, or insulin. Animal component-free media do not contain any animal- or human-derived components. Elimination of these components further improves culture consistency compared to xeno-free media.

Benefits of Serum-Free Cell Culture

Using serum-free media eliminates variability from trace animal components in your culture media and reduces the risk of inconsistent results. Cultures expanded in these media are unaffected by the presence of unknown levels of vitamins, hormones, and growth factors in serum. Read The Importance of Serum- and Animal-Free Media for Stem Cell-Based Therapeutics Article at RegMedNet.

Cell Culture Media