Detects feline IL-10 in direct ELISAs and Western blots. In direct ELISAs, 40% cross‑reactivity with canine IL‑10 is observed and no cross‑reactivity with IL-10 from human, mouse, rat, Epstein-Barr virus, or human cytomegalovirus is observed.
Monoclonal Mouse IgG1 Clone # 137706
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant feline IL‑10 Ser19-Ile178 Accession # P55029
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 2 ng/mL Recombinant Feline IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑10 and Neutralization by Feline IL‑10 Antibody. Recombinant Feline IL‑10 (Catalog # 736-FL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL‑10 Monoclonal Antibody (Catalog # MAB736). The ND50 is typically 0.2-0.6 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The feline homologue of murine IL-10 has been cloned. Feline IL-10 shares 78% amino acid sequence homology with human IL-10 and 73% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.
Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
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