GFP Antibody
GFP Antibody Summary
Ser2-Lys238
Accession # P42212
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of GFP by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with eGFP-tagged EDG6. PVDF membrane was probed with 1 µg/mL of Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GFP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GFP in HEK293 human embryonic kidney cells transfected with GFP. GFP was detected in immersion fixed HEK293 human embryonic kidney cells transfected with GFP (green) using Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, middle panel; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of GFP-positive cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of GFP in HEK293 human embryonic kidney cells transfected with GFP. HEK293 human embryonic kidney cells transfected with GFP was stained with Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody only (A, Catalog # F0108) or with Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by Secondary Antibody (B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
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Detection of GFP by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line either mock transfected (-) or transfected with eGFP-tagged EDG6 (+), loaded at 0.2 mg/mL. A specific band was detected for GFP at approximately 114 kDa (as indicated) using 2.5 µg/mL of Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFP
Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.
Product Datasheets
Citations for GFP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
International Journal of Molecular Sciences, 2022;23(2):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Neonatal diabetes mutations disrupt a chromatin pioneering function that activates the human insulin gene
Authors: I Akerman, MA Maestro, E De Franco, V Grau, S Flanagan, J García-Hur, G Mittler, P Ravassard, L Piemonti, S Ellard, AT Hattersley, J Ferrer
Cell Reports, 2021;35(2):108981.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation
Authors: D Dora, S Ferenczi, R Stavely, VE Toth, ZV Varga, T Kovacs, I Bodi, R Hotta, KJ Kovacs, AM Goldstein, N Nagy
Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause
Authors: R Fan, YS Kim, J Wu, R Chen, D Zeuschner, K Mildner, K Adachi, G Wu, S Galatidou, J Li, HR Schöler, SA Leidel, I Bedzhov
Nat Commun, 2020;11(1):5499.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells
Authors: J Cendrowski, M Kaczmarek, M Mazur, K Kuzmicz-Ko, K Jastrzebsk, M Brewinska-, A Kominek, K Piwocka, M Miaczynska
Elife, 2020;9(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-IP -
Agrin has a pathological role in the progression of oral cancer
Authors: C Rivera, FS Zandonadi, C Sánchez-Ro, CD Soares, DC Granato, WA González-A, AF Paes Leme
Br. J. Cancer, 2018;0(0):.
Applications: Immunoprecipitation -
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
PLoS ONE, 2017;12(6):e0179257.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
FAQs
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Reconstitution Buffers
Secondary Antibodies
Reviews for GFP Antibody
Average Rating: 4 (Based on 3 Reviews)
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