Detection of Glutathione S‑Transferase/GST by Western Blot.
Western blot shows lysates of GST tagged ISG-15, GST tagged Ubiquitin, GST tagged FLAG®, HEK293T human embryonic kidney cell line, E. coli transfected with GST tagged BRDG1-SH2, and Sf 21 transfected GST tagged PARK1. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB9499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S‑Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
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