GSK-3 beta Antibody - BSA Free

Name: GSK-3 beta Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-31353
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-31353

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (98%), Porcine (90%), Sheep (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all GSK-3 beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSK-3 beta. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%). Xenopus laevis (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 beta Antibody - BSA Free

Western Blot: GSK-3 beta Antibody [NBP1-31353]

Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody [NBP1-31353]

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta stained by GSK3 beta antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - H661 xenograft, using GSK3 beta antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: GSK-3 beta Antibody [NBP1-31353]

Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:3000.

Western Blot: GSK-3 beta Antibody [NBP1-31353]

Western Blot: GSK-3 beta Antibody [NBP1-31353] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3].

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody [NBP1-31353]

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Rat brain. GSK3 beta antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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Applications for GSK-3 beta Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GSK-3 beta

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Gene Symbol

GSK3B

Additional GSK-3 beta Products

Product Documents for GSK-3 beta Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for GSK-3 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.