Hematopoietic Stem Cell Lineage Depletion Kit & Individual Antibodies

To enrich for hematopoietic stem cells through negative selection.

Product Summary

The MagCellect Mouse Cell Lineage Depletion Kit (Catalog # MAGM209) uses an antibody cocktail to magnetically remove lineage committed cells such as T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting lineage negative HSCs are untouched by antibodies and ready for use in downstream applications. R&D Systems also offers individual antibodies for lineage depletion which can be used in combination with a magnetic separation system or flow cytometric cell sorting to deplete lineage committed cells.


  • Use of several antibodies increases purity
  • Negative selection reduces sources of experimental error
  • Negatively selected cells are untouched by beads or antibodies

View Product Details, Data Examples, & Protocols:

Lineage Depletion Kit

MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit

To isolate untouched, lineage negative cells from mouse bone marrow.

Individual Monoclonal Antibodies for Lineage Depletion

Lineage Marker Used to Deplete
B220/CD45 B cells
CD3 T cells
CD4 T cells
CD5 B cells (IgM-secreting)
CD8 alpha Cytotoxic T cells and Dendritic cells
Integrin alpha M/CD11b Granulocytes, macrophages, dendritic cells, NK cells
Gr-1/Ly-6G Monocytes and neutrophils
TER-119 Erythrocytes