To enrich for hematopoietic stem cells through negative selection.
The MagCellect Mouse Cell Lineage Depletion Kit (Catalog # MAGM209) uses an antibody cocktail to magnetically remove lineage committed cells such as T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting lineage negative HSCs are untouched by antibodies and ready for use in downstream applications. R&D Systems also offers individual antibodies for lineage depletion which can be used in combination with a magnetic separation system or flow cytometric cell sorting to deplete lineage committed cells.
Use of several antibodies increases purity
Negative selection reduces sources of experimental error
Negatively selected cells are untouched by beads or antibodies