To enrich for hematopoietic stem cells through negative selection.
Product Summary
The MagCellect Mouse Cell Lineage Depletion Kit (Catalog # MAGM209) uses an antibody cocktail to magnetically remove lineage committed cells such as T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting lineage negative HSCs are untouched by antibodies and ready for use in downstream applications. R&D Systems also offers individual antibodies for lineage depletion which can be used in combination with a magnetic separation system or flow cytometric cell sorting to deplete lineage committed cells.
Features
- Use of several antibodies increases purity
- Negative selection reduces sources of experimental error
- Negatively selected cells are untouched by beads or antibodies
View Product Details, Data Examples, & Protocols:
Lineage Depletion Kit
MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit
To isolate untouched, lineage negative cells from mouse bone marrow.
Individual Monoclonal Antibodies for Lineage Depletion
| Lineage Marker | Used to Deplete |
|---|---|
| B220/CD45 | B cells |
| CD3 | T cells |
| CD4 | T cells |
| CD5 | B cells (IgM-secreting) |
| CD8 alpha | Cytotoxic T cells and Dendritic cells |
| Integrin alpha M/CD11b | Granulocytes, macrophages, dendritic cells, NK cells |
| Gr-1/Ly-6G | Monocytes and neutrophils |
| TER-119 | Erythrocytes |