HIV-1 Gag p24 Antibody

Catalog # Availability Size / Price Qty
Detection of HIV-1 Gag p24 by Western Blot.
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Product Details
Citations (3)
Supplemental Products
Reviews (2)

HIV-1 Gag p24 Antibody Summary

Species Reactivity
Detects viral HIV-1 Gag p24 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 749140
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant viral HIV-1 Gag p24
Accession # NP_057850
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of HIV-1 Gag p24 antibody by Western Blot. View Larger

Detection of HIV-1 Gag p24 by Western Blot. Western blot shows recombinant HIV Gag p24 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB7360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs
155030 (Viral)
Alternate Names
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)

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Citations for HIV-1 Gag p24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential
    Authors: B Meyer, J Chiaravall, S Gellenonco, P Brownridge, DP Bryne, LA Daly, A Grauslys, M Walter, F Agou, LA Chakrabart, CS Craik, CE Eyers, PA Eyers, Y Gambin, AR Jones, E Sierecki, E Verdin, M Vignuzzi, E Emmott
    Nature Communications, 2021-09-21;12(1):5553.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis
    Authors: M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane
    PloS Pathogens, 2021-04-19;17(4):e1009526.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Hydroxyproline-containing collagen peptide derived from the skin of the Alaska pollack inhibits HIV-1 infection
    Mol Med Rep, 2016-11-16;14(6):5489-5494.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for HIV-1 Gag p24 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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HIV-1 Gag p24 Antibody
By Anonymous on 10/09/2018
Application: WB Sample Tested: Cell Lysates Species: HIV

gag protein w/o HIV protease, expressed in HEK293 cells, 1/500 dilution

HIV-1 Gag p24 Antibody
By Anonymous on 02/24/2016
Application: ELISA Sample Tested: Cell Lysates Species: Viral