Human ADAM10 Ectodomain Antibody
Human ADAM10 Ectodomain Antibody Summary
Thr214-Glu672
Accession # O14672
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM10
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
- Rooke, et al. (1996) Science 273:1227.
- Pan and Rubin (1997) Cell 90:271.
- Qi, et al. (1999) Science 283:91.
- Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
- Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
- Chantry, et al. (1989) J. Biol. Chem. 264:21603.
- Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
- Chantry and Glynn (1990) Biochem. J. 268:245.
- Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Product Datasheets
Citations for Human ADAM10 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Isolation and characterization of extracellular vesicle subpopulations from tissues
Authors: R Crescitell, C Lässer, J Lötvall
Nature Protocols, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
S. aureus alpha-toxin monomer binding and heptamer formation in host cell membranes - Do they determine sensitivity of airway epithelial cells toward the toxin?
Authors: N Möller, S Ziesemer, P Hildebrand, N Assenheime, U Völker, JP Hildebrand
PLoS ONE, 2020;15(5):e0233854.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Non-cell-autonomous function of DR6 in Schwann cell proliferation
Authors: A Colombo, HE Hsia, M Wang, PH Kuhn, MS Brill, P Canevazzi, R Feederle, C Taveggia, T Misgeld, SF Lichtentha
EMBO J., 2018;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17
Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz
J. Biol. Chem, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
J Immunol, 2015;195(2):736-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).
Authors: Breshears L, Schlievert P, Peterson M
J Biol Chem, 2012;287(39):32578-87.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
Blood, 2009;113(19):4799-809.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
Cell, 2005;123(2):291-304.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
Am. J. Respir. Cell Mol. Biol., 2004;31(4):413-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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