Detects human ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ADAM10, approximately 5% cross-reactivity with recombinant human (rh) TACE, and less than 1% cross-reactivity with rhADAM8, rhADAM9, rhADAM15, and rhBACE is observed.
Polyclonal Goat IgG
Protein A or G purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM10 Thr214-Glu672 Accession # O14672
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Recombinant Human ADAM10 (Catalog # 936-AD) under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ADAM10 in Human Prostate Cancer Tissue. ADAM10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human ADAM10 Ectodomain Polyclonal Antibody (Catalog # AB936) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, and myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine, and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
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Pan and Rubin (1997) Cell 90:271.
Qi, et al. (1999) Science 283:91.
Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
Chantry, et al. (1989) J. Biol. Chem. 264:21603.
Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
Chantry and Glynn (1990) Biochem. J. 268:245.
Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs:
102 (Human); 11487 (Mouse)
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 22.214.171.124; Mammalian disintegrin-metalloprotease
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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