Human ADAM10 Ectodomain Antibody Summary
Accession # O14672
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ADAM10 in Human Prostate Cancer Tissue. ADAM10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human ADAM10 Ectodomain Polyclonal Antibody (Catalog # AB936) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, and myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine, and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
- Rooke, et al. (1996) Science 273:1227.
- Pan and Rubin (1997) Cell 90:271.
- Qi, et al. (1999) Science 283:91.
- Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
- Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
- Chantry, et al. (1989) J. Biol. Chem. 264:21603.
- Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
- Chantry and Glynn (1990) Biochem. J. 268:245.
- Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Citations for Human ADAM10 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Serological immune response against ADAM10 pro-domain is associated with favourable prognosis in stage III colorectal cancer patients
Authors: SM Álvarez-Fe, M Barbariga, L Cannizzaro, CV Cannistrac, L Hurley, A Zanardi, A Conti, F Sanvito, A Innocenzi, N Pecorelli, M Braga, M Alessio
Sample Types: Serum
Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
PLoS ONE, 2012;7(5):e37917.
Sample Types: Cell Lysates
Applications: Western Blot
Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma.
Authors: Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N
Cancer Res., 2009;69(20):8050-7.
Sample Types: Whole Tissue
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