Human ADAM15 Ectodomain Antibody

Catalog # Availability Size / Price Qty
ADAM15 in Human Prostate.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human ADAM15 Ectodomain Antibody Summary

Species Reactivity
Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Ectodomain
Accession # Q13444
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
5-15 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ADAM15 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name
A Disintegrin and Metalloprotease-like Domain 15
Entrez Gene IDs
8751 (Human); 11490 (Mouse)
Alternate Names
ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin

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Citations for Human ADAM15 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts
    Authors: T Janczi, F Meier, Y Fehrl, RW Kinne, B Böhm, H Burkhardt
    Cells, 2021;10(10):.
  2. Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins.
    Authors: Kleino I, Ortiz RM, Yritys M, Huovila AP, Saksela K
    J. Cell. Biochem., 2009;108(4):877-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ADAM15 upregulation and interaction with multiple binding partners in inflammatory bowel disease.
    Authors: Mosnier JF, Jarry A, Bou-Hanna C, Denis MG, Merlin D, Laboisse CL
    Lab. Invest., 2006;86(10):1064-73.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Reviews for Human ADAM15 Ectodomain Antibody

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Human ADAM15 Ectodomain Antibody
By Anonymous on 09/30/2018
Application: ICC/IF Sample Tested: Adult lung Species: Human

Thank you so much for taking the time to share your experience using RnD Systems Antibodies. We value your opinion and use the online reviews as testimonials from our customers, of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when customers report they have not had success. In our QC process, ADAM15 staining was detected in paraffin-embedded sections of human prostate using # AF935 at 5 µg/mL overnight at 4 °C with Anti-Goat HRP-DAB Cell Tissue Staining Kit Catalog # CTS008, using Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. Customer have tested AF935 and MAB935 in their staining. MAB935 works in human lung section and customer doesn’t need further troubleshooting.