Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ADAM15 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15 Ectodomain
Leu18-Ser693
Accession # Q13444

Specificity

Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ADAM15 Ectodomain Antibody

ADAM15 antibody in Human Prostate by Immunohistochemistry (IHC-P).

ADAM15 in Human Prostate.

ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ADAM15 Ectodomain Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample:  Immersion fixed paraffin-embedded sections of human prostate

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)

Reviewed Applications

Read 1 review rated 2 using AF935 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Entrez Gene IDs

8751 (Human); 11490 (Mouse)

Gene Symbol

ADAM15

UniProt

Q13444

Additional ADAM15 Products

Product Documents for Human ADAM15 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAM15 Ectodomain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAM15 Ectodomain Antibody

Customer Reviews for Human ADAM15 Ectodomain Antibody (1)

2 out of 5
1 Customer Rating
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Customer Images

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  • Human ADAM15 Ectodomain Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 09/30/2018
    Human ADAM15 Ectodomain Antibody AF935
    Bio-Techne Response
    Thank you so much for taking the time to share your experience using RnD Systems Antibodies. We value your opinion and use the online reviews as testimonials from our customers, of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when customers report they have not had success. In our QC process, ADAM15 staining was detected in paraffin-embedded sections of human prostate using # AF935 at 5 µg/mL overnight at 4°C with Anti-Goat HRP-DAB Cell Tissue Staining Kit Catalog # CTS008, using Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. Customer have tested AF935 and MAB935 in their staining. MAB935 works in human lung section and customer doesn't need further troubleshooting.

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Protocols

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