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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 498805
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM22
Gly26-His528
Accession # Q9P0K1
Gly26-His528
Accession # Q9P0K1
Specificity
Detects human ADAM22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ADAM8, 9, 10, 12, 15, 19, 23, 32, or 33 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ADAM22 Antibody (498805)
Detection of Human ADAM22 by Western Blot.
Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, Jurkat human acute T cell leukemia cell line (negative control), and HepG2 human hepatocellular carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAM22 Monoclonal Antibody (Catalog # MAB49081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for ADAM22 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ADAM22 Antibody (498805)
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM22, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM22, see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Human brain (motor cortex) tissue and Human brain (cerebellum) tissue
Sample: Human brain (motor cortex) tissue and Human brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ADAM22
Long Name
A Disintegrin and Metalloproteinase Domain 22
Alternate Names
MDC2
Gene Symbol
ADAM22
UniProt
Additional ADAM22 Products
Product Documents for Human ADAM22 Antibody (498805)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ADAM22 Antibody (498805)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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