Human ADAM22 Antibody

Catalog # Availability Size / Price Qty
Detection of Human ADAM22 by Western Blot.
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Product Details
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Human ADAM22 Antibody Summary

Species Reactivity
Detects human ADAM22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ADAM8, 9, 10, 12, 15, 19, 23, 32, or 33 is observed.
Monoclonal Mouse IgG2B Clone # 498805
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ADAM22
Accession # Q9P0K1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM22, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ADAM22 antibody by Western Blot. View Larger

Detection of Human ADAM22 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, Jurkat human acute T cell leukemia cell line (negative control), and HepG2 human hepatocellular carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAM22 Monoclonal Antibody (Catalog # MAB49081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for ADAM22 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM22

Human ADAM22 is a member of the ADAM (a disintegrin and metalloprotease domain) family of metalloproteases. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins. They have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, such as fertilization, muscle development, and neurogenesis. ADAM22 is highly expressed in the brain and may function as an integrin ligand in the brain. The amino acid sequence of human ADAM22 is 100%, 96% and 92% identical to that of chimpanzee, dog and mouse.

Long Name
A Disintegrin and Metalloproteinase Domain 22
Entrez Gene IDs
53616 (Human); 11496 (Mouse); 57033 (Rat)
Alternate Names
ADAM 22; ADAM metallopeptidase domain 22; ADAM22; MDC2; metalloproteinase-disintegrin ADAM22-3; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2; MGC149832

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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