Human Adrenomedullin R/ADMR Antibody Summary
Accession # O15218
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Adrenomedullin R/ADMR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Adrenomedullin R/ADMR in Human Spleen Tissue. Adrenomedullin R/ADMR was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cell in sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adrenomedullin R/ADMR
ADMR (GPR182) is a seven transmembrane orphan receptor originally thought to be a receptor for adrenomedullin. ADMR is highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. Expression is in endothelial cells along the vascular tree.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human Adrenomedullin R/ADMR Antibody
There are currently no reviews for this product. Be the first to review Human Adrenomedullin R/ADMR Antibody and earn rewards!
Have you used Human Adrenomedullin R/ADMR Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image