Human Adrenomedullin R/ADMR Antibody

Catalog # Availability Size / Price Qty
MAB10293-100
MAB10293-SP
Human Adrenomedullin R/ADMR Antibody in Flow Cytometry
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Human Adrenomedullin R/ADMR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adrenomedullin R/ADMR in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 528563
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Adrenomedullin R/ADMR
Accession # O15218
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry. View Larger

Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Adrenomedullin R/ADMR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Adrenomedullin  R/ADMR in Human Spleen Tissue. View Larger

Adrenomedullin R/ADMR in Human Spleen Tissue. Adrenomedullin R/ADMR was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cell in sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin R/ADMR

ADMR (GPR182) is a seven transmembrane orphan receptor originally thought to be a receptor for adrenomedullin. ADMR is highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. Expression is in endothelial cells along the vascular tree.

Long Name
Adrenomedullin Receptor
Entrez Gene IDs
11318 (Human); 11536 (Mouse); 29307 (Rat)
Alternate Names
7TMR; ADMR; Adrenomedullin R; adrenomedullin receptor; AdrenomedullinR; AMR; AM-R; G protein-coupled receptor 182,7TMR; G10D; GAMRH; Gpcr22; GPR182; G-protein coupled receptor 182; hrhAMR; MB10; MGC34399; NOW

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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