Human ALK/CD246 Antibody

Catalog # Availability Size / Price Qty
MAB42101-100
MAB42101-SP
ALK/CD246  in SH-SY5Y Human Cell Line.
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Human ALK/CD246 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALK/CD246 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 383603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALK/CD246
Val19-Ser1038
Accession # Q0UM73
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ALK/CD246  in SH-SY5Y Human Cell Line. View Larger

ALK/CD246 in SH-SY5Y Human Cell Line. ALK/CD246 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Mouse Anti-Human ALK/CD246 Monoclonal Antibody (Catalog # MAB42101) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK/CD246

Anaplastic Lymphoma Kinase (ALK), also known as CD246, is a 220 kDa transmembrane glycoprotein that is primarily expressed in the developing nervous system. ALK contains one LDLR class A domain, two MAM domains, and a cytoplasmic kinase domain. Chromosomal translocation involving ALK and the nucleolar NPM protein result in a cytoplasmic fusion protein that exists as a dimer. The kinase domain in NPM-ALK is constitutively active and participates in cellular transformation. ALK translocations are found in a variety of tumors. Within the extracellular domain, human and mouse ALK share 88% amino acid sequence identity.

Long Name
Anaplastic Lymphoma Kinase
Entrez Gene IDs
238 (Human); 11682 (Mouse); 266802 (Rat)
Alternate Names
ALK tyrosine kinase receptor; ALK; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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