Human Alkaline Phosphatase/ALPP Antibody (696108)
R&D Systems | Catalog # MAB5905
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 696108
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Alkaline Phosphatase/ALPP
Ile23-Asp506
Accession # P05187
Ile23-Asp506
Accession # P05187
Specificity
Detects human Alkaline Phosphatase/ALPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Intestinal Alkaline Phosphatase/ALPI or rhAlkaline Phsophatase/ALPL is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Alkaline Phosphatase/ALPP Antibody (696108)
Detection of Human Alkaline Phosphatase/ALPP by Western Blot.
Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB5905) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Alkaline Phosphatase/ALPP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Alkaline Phosphatase/ALPP Antibody (696108)
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Alkaline Phosphatase/ALPP, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Alkaline Phosphatase/ALPP, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: JAR human choriocarcinoma cell line
Sample: JAR human choriocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
UniProt
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Human Alkaline Phosphatase/ALPP Antibody (696108)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Alkaline Phosphatase/ALPP Antibody (696108)
For research use only
Related Research Areas
Citations for Human Alkaline Phosphatase/ALPP Antibody (696108)
Powered by Bioz
Customer Reviews for Human Alkaline Phosphatase/ALPP Antibody (696108)
There are currently no reviews for this product. Be the first to review Human Alkaline Phosphatase/ALPP Antibody (696108) and earn rewards!
Have you used Human Alkaline Phosphatase/ALPP Antibody (696108)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...