Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 257303
View all alpha 2-Macroglobulin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived alpha 2-Macroglobulin

Specificity

Detects human alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha 2‑Macroglobulin Antibody

Human alpha 2‑Macroglobulin ELISA Standard Curve.

Recombinant Human alpha 2‑Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human alpha 2‑Macroglobulin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha 2-Macroglobulin DuoSet ELISA Kit (Catalog # DY1938) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 2-Macroglobulin

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Entrez Gene IDs

2 (Human)

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Human alpha 2‑Macroglobulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human alpha 2‑Macroglobulin Antibody

For research use only

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