Human alpha -Defensin 5 Antibody

Catalog # Availability Size / Price Qty
MAB102481-100
MAB102481-SP
Human  alpha -Defensin 5 Antibody in ELISA
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Human alpha -Defensin 5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Defensin 5 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 972217
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human alpha -Defensin 5
Thr64-Arg94
Accession # Q01523
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB10248).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human alpha -Defensin 5 ELISA Standard Curve. Recombinant Human alpha -Defensin 5 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB10248) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB102481) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Defensin 5

Defensin alpha 5 (DEFA5), also known as human alpha defensin 5 (HD5), is a secreted antimicrobial protein encoded by the DEFA5 gene and a member of the Defensins family. Defensins are thought to kill microbes by permeabilizing their plasma membrane. DEFA5 is expressed in Paneth cells, which are secretory epithelial cells in the small intestine. Unlike other defensins, DEFA5 is stored in secretory vesicles as a propeptide, and is processed into different peptides with different potency and localization.

Long Name
Defensin Alpha 5
Entrez Gene IDs
1670 (Human)
Alternate Names
alpha-Defensin 5; DEF5; DEF5defensin, alpha 5, preproprotein; DEFA5; defensin 5; Defensin alpha 5; Defensin, alpha 5; defensin, alpha 5, Paneth cell-specific; defensin-5; HD-5; MGC129728

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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