Human alpha -Defensin 5 Antibody Summary
Accession # Q01523
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB10248).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human alpha -Defensin 5 ELISA Standard Curve. Recombinant Human a-Defensin 5 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB10248) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB102481) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Defensin 5
Defensin alpha 5 (DEFA5), also known as human alpha defensin 5 (HD5), is a secreted antimicrobial protein encoded by the DEFA5 gene and a member of the Defensins family. Defensins are thought to kill microbes by permeabilizing their plasma membrane. DEFA5 is expressed in Paneth cells, which are secretory epithelial cells in the small intestine. Unlike other defensins, DEFA5 is stored in secretory vesicles as a propeptide, and is processed into different peptides with different potency and localization.
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