Human Angiopoietin-2 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, or rmANGPTL3.
  • Source
    Monoclonal Mouse IgG2B Clone # 180102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-2
    Asp68-Phe496
    Accession # O15123
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Angiopoietin‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, human placenta tissue, human ovary tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Angiopoietin‑2 Monoclonal Antibody (Catalog # MAB0983) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin‑2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166 - 248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275 - 495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65 - 66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250 - 300 kDa tetramers and pentamers (3 - 6). Alternate splicing generates a short isoform that lacks 52 amino acids preceding the coiled-coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang‑1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang‑2 becomes as a Tie-2 agonist (3, 8 - 11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17 - 20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

  • References:
    1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
    2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
    3. Maisonpierre, P.C. et al. (1997) Science 27:55.
    4. Kim, I. et al. (2000) J. Biol. Chem. 275:18550.
    5. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
    6. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
    7. Gale, N.W. et al. (2002) Dev. Cell 3:411.
    8. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
    9. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
    10. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
    11. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
    12. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
    13. Cao, Y. et al. (2007) Cancer Res. 67:3835.
    14. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
    15. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
    16. Fiedler, U. et al. (2006) Nat. Med. 12:235.
    17. Koga, K. et al. (2001) Cancer Res. 61:6248.
    18. Etoh, T. et al. (2001) Cancer Res. 61:2145.
    19. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
    20. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
    21. Hu, B. et al. (2006) Cancer Res. 66:775.
    22. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.
  • Entrez Gene IDs:
    285 (Human); 11601 (Mouse)
  • Alternate Names:
    AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Regulation of angiopoietin expression by bacterial lipopolysaccharide.
    Authors: Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L955-63.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
    Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L142-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
    Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
    Am. J. Obstet. Gynecol., 2006;194(2):564-71.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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