Human Angiopoietin-like Protein 8/Betatrophin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Angiopoietin-like Protein 8/Betatrophin in direct ELISA and Western Blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 910248
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human Angiopoietin-like Protein 8/Betatrophin synthetic peptide with sequences corresponding to the N-term region
    Accession # Q6UXH0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

Angiopoietin-like Protein 8/Betatrophin in Human Liver.
Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB8548) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Angiopoietin-like Protein 8/Betatrophin by Western Blot.
Western blot shows lysates of CHO Chinese hamster ovary cell line and HEK293 human embryonic kidney cell line either mock transfected or transfected with human Angiopoietin-like Protein 8 (ANGPTL8)/Betatrophin. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Polyclonal Antibody (Catalog # MAB8548) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-like Protein 8/Betatrophin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Angiopoietin-like Protein 8/Betatrophin in HepG2 Human Cell Line. Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Monoclonal Antibody (Catalog # MAB8548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 8/Betatrophin
Betatrophin also known as C19orf80, ANGPTL8, and TD26, is a secreted hormone protein.  It is 198 amino acids (aa) in length, and shares 72% aa identity with mouse Betatrophin.  It is primarily expressed in the liver and adipose tissue and has been shown to be increased in the plasma of diabetic patients.  Additionally, Betatrophin has been demonstrated to increase the proliferation of beta cells in the pancreas of mice.
  • Entrez Gene IDs:
    55908 (Human); 624219 (Mouse); 100361444 (Rat)
  • Alternate Names:
    Angiopoietin like Protein 8; Angiopoietin-like Protein 8; ANGPTL8; Betatrophin; C19orf80; chromosome 19 open reading frame 80; Gm6484; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; Lipasin; PRO1185; PVPA599; RIFL; TD26; UNQ599
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