Human Angiopoietin-like Protein 8/Betatrophin Antibody Summary
Accession # Q6UXH0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Angiopoietin-like Protein 8/Betatrophin in Human Liver. Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB8548) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Angiopoietin-like Protein 8/Betatrophin by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line and HEK293 human embryonic kidney cell line either mock transfected or transfected with human Angiopoietin-like Protein 8 (ANGPTL8)/Betatrophin. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Polyclonal Antibody (Catalog # MAB8548) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-like Protein 8/Betatrophin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Angiopoietin-like Protein 8/Betatrophin in HepG2 Human Cell Line. Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Monoclonal Antibody (Catalog # MAB8548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 8/Betatrophin
Betatrophin also known as C19orf80, ANGPTL8, and TD26, is a secreted hormone protein. It is 198 amino acids (aa) in length, and shares 72% aa identity with mouse Betatrophin. It is primarily expressed in the liver and adipose tissue and has been shown to be increased in the plasma of diabetic patients. Additionally, Betatrophin has been demonstrated to increase the proliferation of beta cells in the pancreas of mice.
Citation for Human Angiopoietin-like Protein 8/Betatrophin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Increased expression level of ANGPTL8 in white adipose tissue under acute and chronic cold treatment
Authors: H Arefanian, I Al-Khairi, NA Khalaf, P Cherian, S Kavalakatt, D Madhu, A Mathur, MG Qaddoumi, F Al-Mulla, J Abubaker, M Abu-Farha
Lipids in Health and Disease, 2021;20(1):117.
Sample Types: Whole Tissue
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