Human Annexin A6 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Annexin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A8, A9, A10, A11, or A13 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 686921
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Annexin A6
    Ala2-Asp673
    Accession # P08133
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Annexin A6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunohistochemistry
Annexin A6 in Human Placenta. Annexin A6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60-70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5‑7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

  • References:
    1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
    2. Crompton, M.R. et al. (1988) EMBO J. 7:21.
    3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
    4. Grewal, T. et al. (2006) Bioessays 28:1211.
    5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
    6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
    7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
    8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.
  • Entrez Gene IDs:
    309 (Human); 11749 (Mouse); 79125 (Rat)
  • Alternate Names:
    67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III
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