Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 686921
View all Annexin A6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Annexin A6
Ala2-Asp673
Accession # P08133

Specificity

Detects human Annexin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A8, A9, A10, A11, or A13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Annexin A6 Antibody

Detection of Human, Mouse, and Rat Annexin A6 antibody by Western Blot.

Detection of Human, Mouse, and Rat Annexin A6 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human Annexin A6 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A6 at approximately 72 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Annexin A6 antibody in Human Placenta by Immunohistochemistry (IHC-P).

Annexin A6 in Human Placenta.

Annexin A6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Annexin A6 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Simple Western

1 µg/mL
Sample: Exosome Standards (LNCaP) (Catalog # NBP3-11687), Exosome Standards (PC-3) (Catalog # NBP2-49856) and Jurkat human acute T cell leukemia cell line

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60-70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5‑7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

References

  1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
  2. Crompton, M.R. et al. (1988) EMBO J. 7:21.
  3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
  4. Grewal, T. et al. (2006) Bioessays 28:1211.
  5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
  6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
  7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
  8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

Alternate Names

ANX6, ANXA6, Calelectrin, CBP68, CPB-II, Lipocortin VI

Entrez Gene IDs

309 (Human); 11749 (Mouse); 79125 (Rat)

Gene Symbol

ANXA6

UniProt

P08133 (Human)

Additional Annexin A6 Products

Product Documents for Human Annexin A6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Annexin A6 Antibody

For research use only

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Protocols

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