Detection of Human APLP‑2 by Western Blot.
Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human APLP‑2 Monoclonal Antibody (Catalog # MAB49451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APLP‑2 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Amyloid beta precursor-like protein 2 (APLP-2) is a 120-170 kDa member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% aa sequence identity with mouse and rat APLP-2, respectively.
Amyloid Beta [A4] Precursor-like Protein 2
Entrez Gene IDs:
334 (Human); 11804 (Mouse); 64312 (Rat)
amyloid beta (A4) precursor-like protein 2; amyloid precursor protein homolog HSD-2; Amyloid protein homolog; amyloid-like protein 2; APLP2; APLP-2; APPH; APPHCDEI box-binding protein; APPL2; CDEBP; WALPLP2; YWK-II
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