Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ATR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ATR
Glu2405-Met2644
Accession # Q13535

Specificity

Detects human ATR in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ATR Antibody

Detection of Human ATR antibody by Western Blot.

Detection of Human ATR by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATR at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ATR antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

ATR in Human Ovarian Cancer Tissue.

ATR was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ATR Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATR

ATR (Ataxia telangiectasia and Rad 3-related) is a member of the phosphatidylinositol kinase-related kinase (PIKK) family of protein kinases. ATR is a large protein kinase (~300 kDa) that functions in the response to genotoxic stress, DNA recombination, and cell cycle control.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Entrez Gene IDs

545 (Human); 245000 (Mouse); 685055 (Rat)

Gene Symbol

ATR

UniProt

Q13535

Additional ATR Products

Product Documents for Human ATR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ATR Antibody

For research use only

Related Research Areas

p53 Pathway

Citations for Human ATR Antibody

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Protocols

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FAQs

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