Human ATR Antibody Summary
Accession # Q13535
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human ATR by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATR at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ATR in Human Ovarian Cancer Tissue. ATR was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ATR (Ataxia telangiectasia and Rad 3-related) is a member of the phosphatidylinositol kinase-related kinase (PIKK) family of protein kinases. ATR is a large protein kinase (~300 kDa) that functions in the response to genotoxic stress, DNA recombination, and cell cycle control.
Citation for Human ATR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Time-resolved analysis of DNA-protein interactions in living cells by UV laser pulses
Authors: A Nebbioso, R Benedetti, M Conte, V Carafa, F De Bellis, J Shaik, F Matarese, B Della Vent, F Gesuele, R Velotta, JHA Martens, HG Stunnenber, C Altucci, L Altucci
Sci Rep, 2017;7(1):11725.
Sample Types: Cell Lysates
Applications: Western Blot
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