Human Axin-2 Antibody (354214)

R&D Systems | Catalog # MAB6078

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 354214
View all Axin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Axin-2
Tyr550-Gln760
Accession # Q9Y2T1

Specificity

Detects human Axin-2 in direct ELISAs.  In this format, no cross-reactivity with recombinant human Axin-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Axin-2 Antibody (354214)

Axin-2 antibody in SW480 Human Cell Line by Immunocytochemistry (ICC).

Axin‑2 in SW480 Human Cell Line.

Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Axin-2 Antibody (354214)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed SW480 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axin-2

Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta -catenin, glycogen synthase kinase-3 (GSK-3 beta ), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.

Long Name

Axis Inhibitor 2

Alternate Names

AXIL, Axin2, Conductin, MGC10366, MGC126582

Entrez Gene IDs

8313 (Human)

Gene Symbol

AXIN2

UniProt

Q9Y2T1

Additional Axin-2 Products

Product Documents for Human Axin-2 Antibody (354214)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Axin-2 Antibody (354214)

For research use only

Citations for Human Axin-2 Antibody (354214)

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