Human Axin-2 Antibody
Human Axin-2 Antibody Summary
Tyr550-Gln760
Accession # Q9Y2T1
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Axin‑2 in SW480 Human Cell Line. Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axin-2
Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta -catenin, glycogen synthase kinase-3 (GSK-3 beta ), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.
Product Datasheets
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