Human Axin-2 Antibody

Catalog # Availability Size / Price Qty
Axin‑2 in SW480 Human Cell Line.
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Product Details
Supplemental Products

Human Axin-2 Antibody Summary

Species Reactivity
Detects human Axin-2 in direct ELISAs.  In this format, no cross-reactivity with recombinant human Axin-1 is observed.
Monoclonal Mouse IgG1 Clone # 354214
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Axin-2
Accession # Q9Y2T1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Axin-2 antibody in SW480 Human Cell Line by Immunocytochemistry (ICC). View Larger

Axin‑2 in SW480 Human Cell Line. Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axin-2

Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta -catenin, glycogen synthase kinase-3 (GSK-3 beta ), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.

Long Name
Axis Inhibitor 2
Entrez Gene IDs
8313 (Human)
Alternate Names
AXIL; axin 2; Axin2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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