Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all B7-H7/HHLA2 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
Met1-Asn344
Accession # Q9UM44

Specificity

Detects human B7-H7/HHLA2 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human B7-H7/HHLA2 Antibody

Detection of Human B7-H7/HHLA2 antibody by Western Blot.

Detection of Human B7-H7/HHLA2 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
B7-H7/HHLA2 antibody in Human Monocytes by Immunocytochemistry (ICC).

B7-H7/HHLA2 in Human Monocytes.

B7-H7/HHLA2 was detected in immersion fixed human monocytes using Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human B7-H7/HHLA2 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed human monocytes

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidny cell line transfected with human B7-H7

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Long Name

B7 Homolog 7

Alternate Names

B7H7, HHLA2

Entrez Gene IDs

11148 (Human); 102137704 (Cynomolgus Monkey)

Gene Symbol

HHLA2

UniProt

Q9UM44

Additional B7-H7/HHLA2 Products

Product Documents for Human B7-H7/HHLA2 Antibody

Certificate of Analysis

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Product Specific Notices for Human B7-H7/HHLA2 Antibody

For research use only

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Protocols

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