Detection of Human B7-H7/HHLA2 by Western Blot.Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
B7-H7/HHLA2 in Human Monocytes. B7-H7/HHLA2 was detected in immersion fixed human monocytes using Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Mager, D.L. et al. (1999) Genomics 59:255.
Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
B7 Homolog 7
Entrez Gene IDs:
B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
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