Human BACH1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human BACH1 by Western Blot.
2 Images
Product Details
Citations (5)
Supplemental Products

Human BACH1 Antibody Summary

Species Reactivity
Detects human BACH1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human BACH1
Accession # O14867
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BACH1 antibody by Western Blot. View Larger

Detection of Human BACH1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BACH1 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

BACH1 in Human Lung Cancer Tissue. BACH1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5776) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACH1

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms non‑covalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Human BACH1 is 736 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 562‑577), and a Leu-zipper domain (aa 585‑607). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. There are two BACH1 splice variants with a 24 and 31 aa substitution for aa 593‑736, respectively. Over aa 133‑513, human BACH1 shares 75% aa identity with mouse BACH1.

Long Name
BTB and CNC homolog 1
Entrez Gene IDs
571 (Human); 12013 (Mouse); 304127 (Rat)
Alternate Names
BACH1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; BTB; HA2303; transcription regulator protein BACH1; transcription regulator protein10BACH-1

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Citations for Human BACH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A Heme-Binding Transcription Factor BACH1 Regulates Lactate Catabolism Suggesting a Combined Therapy for Triple-Negative Breast Cancer
    Authors: J Padilla, BS Lee, K Zhai, B Rentz, T Bobo, NM Dowling, J Lee
    Cells, 2022;11(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis
    Authors: Y Zhao, J Gao, X Xie, P Nan, F Liu, Y Sun, X Zhao
    Cancer Medicine, 2021;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  3. Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion
    Authors: J Xu, H Guo, Z Xing, W Zhang, J He, J Cheng, R Cai
    Oxidative Medicine and Cellular Longevity, 2020;2020(0):6240125.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells
    Authors: X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng
    Sci Adv, 2019;5(3):eaau7887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Induction of heme oxygenase I (HMOX1) by HPP-4382: a novel modulator of Bach1 activity.
    Authors: Attucks, Otis C, Jasmer, Kimberly, Hannink, Mark, Kassis, Jareer, Zhong, Zhenping, Gupta, Suparna, Victory, Sam F, Guzel, Mustafa, Polisetti, Dharma R, Andrews, Robert, Mjalli, Adnan M, Kostura, Matthew
    PLoS ONE, 2014;9(7):e101044.
    Species: Human
    Sample Types: Nuclear Extract
    Applications: Immunoprecipitation


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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