|Detection of Human Bag‑1 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag‑1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Bag‑1 at approximately 36 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.|
|Immunoprecipitation of Human Bag‑1. Bag‑1 was immunoprecipitated from lysates (5 x 106 cells) of THP‑1 human acute monocytic leukemia and HEK293 human embryonic kidney cell line following incubation with 3 µg Mouse Anti-Human Bag‑1 Monoclonal Antibody (Catalog # MAB852) for 1-16 hours at 4 °C. Bag‑1-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated Bag‑1 (left panel) was detected by Western blot using 0.5 µg/mL Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852). View our recommended buffer recipes for immunoprecipitation.|
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