Detects human BCAM in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP‑2 is observed.
Monoclonal Mouse IgG2A Clone # 87207
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human BCAM Glu32-Ala547 Accession # CAA58449
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human BCAM Fc Chimera (Catalog # 148-BC)
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Detection of BCAM in Huh‑7 Human Cell Line by Flow Cytometry. Huh‑7 human hepatoma cell line was stained with Mouse Anti-Human BCAM Monoclonal Antibody (Catalog # MAB1481, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig-like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.
Campbell, I.G. et al. (1994) Cancer Research 54:5761.
Parsons, S.F. et al. (1995) Proc. Natl., Acad. Sci. USA, 92:5496.
Udani, M. et al. (1998) 101:2550.
Schon, M. et al. (2000) J. Invest. Dermatol, 115:1047.
Basal Cell Adhesion Molecule
Entrez Gene IDs:
4059 (Human); 57278 (Mouse)
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.