Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Product Details
Supplemental Products

Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody Summary

Species Reactivity
Detects human beta -1,4‑Galactosyltransferase 1/B4GalT1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 394706
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human beta -1,4‑Galactosyltransferase 1/B4GalT1 long isoform
Accession # P15291
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human beta 4GalT1/ beta ‑1,4-Galactosyltransferase 1 (Catalog # 3609-GT)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human beta 4GalT1/
beta ‑1,4-Galactosyltransferase 1 (Catalog # 3609-GT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Galactosyltransferase 1/B4GalT1

beta 4GalT1 is one of seven beta 1,4 galactosyltransferases that transfer galactose in a beta 1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. By sequence similarity, the beta 4GalTs form four groups: beta 4GalT1 and beta 4GalT2, beta 4GalT3 and beta 4GalT4, beta 4GalT5 and beta 4GalT6, and beta 4GalT7 (1). beta 4GalT1 is unique among the seven enzymes because it can be expressed either as membrane associated form or secreted form (2). The secreted form is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha -lactalbumin to catalyze the synthesis of lactose (3). The membrane form can reside either in the Golgi apparatus, where it adds galactose to N-acetylglucosamine residues, or on cell surface, where it functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix (4). The two enzymatic forms result from alternate transcription initiation sites and post-translational processing (5). Defects in beta 4GalT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) (6). The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.

  1. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473:35.
  2. Yamaguchi, N and Fukuda, M.N. (1995) J. Biol. Chem. 270:12170.
  3. Appert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138:224.
  4. Lopez, L.C. et al. (1991) J. Biol. Chem. 266:15984.
  5. Mengle-Gaw, L. et al. (1991) Biochem. Biophys. Res. Commun. 176:1269.
  6. Hansske, B. et al. (2002) J. Clin. Invest. 109:725.
Long Name
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 1
Entrez Gene IDs
2683 (Human); 14595 (Mouse); 24390 (Rat)
Alternate Names
1,4- galactosyltransferase, polypeptide 1; B4GalT1; B4GAL-T1; beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; beta4Gal-T1; betaGlcNAc beta; CDG2D; EC 2.4.1; GGTB2; GGTB2DKFZp686N19253; glycoprotein-4-beta-galactosyltransferase 2; GT1; GTB; lactose synthase; MGC50983; UDP-Gal; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody

There are currently no reviews for this product. Be the first to review Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody and earn rewards!

Have you used Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review